connecting you to a world of zebrafish revertible mutants

Analysis of the zebrafish smoothened mutant reveals

RESULTS Identification of smo mutants and characterization of the smo gene. In an ongoing large-scale insertional mutagenesis screen (Amsterdam et al., 1999), we found two insertional mutant alleles in the zebrafish smo gene.One was found in F 2 family 229, the other in family 1640. We named the mutant smo, and the two alleles smo hi229 and smo hi1640, according to the Zebrafish Nomenclature

Andrew PETZOLD Professor (Assistant) PhD University

The attributes of the zebrafish (Danio rerio) make it an excellent model system for the development and discovery of new drugs. Connecting you to a world of zebrafish revertible mutants Andrew PETZOLD Professor (Assistant) PhD University zfishbook is an internet-based openly accessible database of revertible protein trap gene-breaking transposon (GBT) insertional mutants in the zebrafish, Danio rerio. In these lines, a monomeric

Connecting you to a world of zebrafish revertible mutants

Connecting you to a world of zebrafish revertible mutants. Home; Lines. MN0001 to MN1000. MN0001 to MN0100. MN0001 to MN0010. We are using gene-breaking transposons to create dominantly labeled mutants in zebrafish (Sivasubbu et al., Mech Dev 2006, (2012) "zfishbook:connecting you to a world of zebrafish revertible mutants" Clark Connecting you to a world of zebrafish revertible mutants The following comments are owned by whomever posted them. This site is not responsible for what they say.

FAIRsharing:biodbcore-000108:zfishbook

zfishbook:connecting you to a world of zebrafish revertible mutants. Clark KJ,Argue DP,Petzold AM,Ekker SC Nucleic Acids Res 2011 View Paper (PubMed) View Publication Faculty Research University of Minnesota RochesterConnecting analytics and curriculum design:The process and outcome of building a tool to browse data relevant to course designers. Journal of Learning Analytics. 1(3):220-240. zfishbook:connecting you to a world of zebrafish revertible mutants. Nucleic Acids Research. 40(Database issue):D907-11. Epub 2011 Nov 8.

Forward Genetic Screening Using Behavioral Tests in

The zebrafish enjoys several advantages over other model organisms. It is small, easy to maintain, prolific, and numerous genetic tools are available for it. For example, forward genetic screens have allowed investigators to identify important genes potentially involved in a variety of functions from embryogenesis to cancer. However, despite its sophisticated behavioral repertoire, behavioral Forward Genetic Screening Using Behavioral Tests in The zebrafish enjoys several advantages over other model organisms. It is small, easy to maintain, prolific, and numerous genetic tools are available for it. For example, forward genetic screens have allowed investigators to identify important genes potentially involved in a variety of functions from embryogenesis to cancer. However, despite its sophisticated behavioral repertoire, behavioral

IJMS Free Full-Text Zebrafish Mutants Carrying Leptin

Leptin, a hormone secreted by peripheral adipose tissues, regulates the appetite in animals. Recently, evidence has shown that leptin also plays roles in behavioral response in addition to controlling appetite. In this study, we examined the potential function of leptin on non-appetite behaviors in zebrafish model. By using genome editing tool of Transcription activator-like effector nuclease International Zebrafish Mutagenic Protein Trap - Stephen Clark, Karl J; Argue, David P; Petzold, Andrew M et al. (2012) zfishbook:connecting you to a world of zebrafish revertible mutants. Nucleic Acids Res 40:D907-11 Comments

International Zebrafish Mutagenic Protein Trap - Stephen

The zebrafish (Danio rerio) is the preeminent non-mammalian vertebrate system for the study of core vertebrate biology and behavior and for the modeling of human disease. Collections of molecularly ch International Zebrafish Mutagenic Protein Trap - Stephen EkkerThe zebrafish (Danio rerio) is the preeminent non-mammalian vertebrate system for the study of core vertebrate biology and behavior and for the modeling of human disease. Collections of molecularly ch

International Zebrafish Mutagenic Protein Trap - Stephen Ekker

This proposal is to complete this molecularly characterized collection, in silico catalog and distribution network of revertible mutations for the preeminent non-mammalian vertebrate, the zebrafish. These dominantly marked mutants lines also represent an ideal substrate for large-scale behavioral and other phenotypic assessment of the genome as a part of the broader initiative to functionally annotate the vertebrate genome using the zebrafish. Learning to Fish with Genetics:A Primer on the Vertebrate Jul 01, 2016 · Box 1:golden mutants and skin pigmentation. An unexpected connection between zebrafish and human genetics came from study of the golden mutant, identified by Streisinger et al., which lacks pigmentation in the skin.Keith Chengs laboratory and collaborators found that a mutation in the slc24a gene, which encodes a cation exchanger important for Ca 2+ cycling (Altimimi and

Protein-Trap Insertional Mutagenesis Uncovers New Genes

Jun 25, 2015 · Zebrafish fras1 mutants are characterized by corresponding blistering in the developing fin folds, though zebrafish grip1 mutants have no overt morphological phenotype . Hmcn1/Fibulin 6 is a highly conserved member of the Fibulin ECM protein family, Protein-Trap Insertional Mutagenesis Uncovers New Genes fras1 and hmcn1 GBT alleles phenocopy known ENU mutants and are revertible. To determine which ZIP loci were required for early zebrafish skin or fin fold development, we bred each eing insertion to homozygosity through a standard inbreeding scheme.

Regulation of gluconeogenesis by Aldo-Keto-Reductase

Nov 03, 2020 · Here, we report on a zebrafish mutant for Aldo-keto-reductase 1a1b (akr1a1b) as a regulator of gluconeogenesis. Adult akr1a1b-/- mutant zebrafish developed fasting hypoglycemia, which was caused by inhibiting phosphoenolpyruvate carboxykinase (PEPCK) eion as rate-limiting enzyme of gluconeogenesis. SCORE Imaging:Specimen in a Corrected Optical Rotational zfishbook:connecting you to a world of zebrafish revertible mutants 8 November 2011 Nucleic Acids Research, Vol. 40, No. D1 Fully automated cellular-resolution vertebrate screening platform with parallel animal processing

SCORE Imaging:Specimen in a Corrected Optical Rotational

zfishbook:connecting you to a world of zebrafish revertible mutants 8 November 2011 Nucleic Acids Research, Vol. 40, No. D1 Fully automated cellular-resolution vertebrate screening platform with parallel animal processing The Zebrafish CreZoo:An Easy-to-Handle Database for Novel zfishbook is an internet-based openly accessible database of revertible protein trap gene-breaking transposon (GBT) insertional mutants in the zebrafish, Danio rerio.

Trapping Cardiac Recessive Mutants via Eion-Based

Rationale:Mutagenesis screening is a powerful genetic tool for probing biological mechanisms underlying vertebrate development and human diseases. However, the increased colony management efforts in Trapping Cardiac Recessive Mutants via Eion-based The first two large-scale mutagenesis screens using ENU as a mutagen generated hundreds of mutants, which laid the foundation for the zebrafish to become a premier animal model. 8, 10 To address the limitations of molecular cloning, mutagens with a sequence tag have been exploited, including either retrovirus- 9, 11 or transposon-based vectors

Trapping Cardiac Recessive Mutants via Eion-based

The first two large-scale mutagenesis screens using ENU as a mutagen generated hundreds of mutants, which laid the foundation for the zebrafish to become a premier animal model. 8, 10 To address the limitations of molecular cloning, mutagens with a sequence tag have been exploited, including either retrovirus- 9, 11 or transposon-based vectors ZFIN Lab:Stephen C. Ekker Laboratory - ZFIN The Zebrafish In parallel, we engineered transposon tools for the zebrafish including protein trap gene-breaking vectors to generate a collection of molecularly characterized and revertible mutant zebrafish lines, an ongoing effort in the laboratory. Custom restriction endonucleases offer a third leg, targeted genetic modification.

ZFIN Publication:Clark et al., 2012

zfishbook is an internet-based openly accessible database of revertible protein trap gene-breaking transposon (GBT) insertional mutants in the zebrafish, Danio rerio. In these lines, a monomeric red fluorescent protein (mRFP) is encoded by an artificial 3' exon, resulting in a translational fusion to endogenous loci. ZFIN Publication:Clark et al., 2012zfishbook is an internet-based openly accessible database of revertible protein trap gene-breaking transposon (GBT) insertional mutants in the zebrafish, Danio rerio. In these lines, a monomeric red fluorescent protein (mRFP) is encoded by an artificial 3' exon, resulting in a translational fusion to endogenous loci.

Zebrafish Behavioral Models of Ageing SpringerLink

Clark KJ, Argue DP, Petzold AM, Ekker SC. zfishbook:connecting you to a world of zebrafish revertible mutants. Nucleic Acids Res. 2012;40(D1):D90711. doi:10.1093/nar/gkr957. PubMed CrossRef Google Scholar Zebrafish Behavioral Models of Ageing SpringerLinkClark KJ, Argue DP, Petzold AM, Ekker SC. zfishbook:connecting you to a world of zebrafish revertible mutants. Nucleic Acids Res. 2012;40(D1):D90711. doi:10.1093/nar/gkr957. PubMed CrossRef Google Scholar

Zebrafish approaches enhance the translational research

Feb 01, 2014 · A number of approaches have been developed to overcome the labor associated with identifying specific genetic mutations resulting from random mutagenesis, including large-scale retroviral insertion approaches.110, 115 The result is a large and growing collection of mutant zebrafish lines, many of which are available for distribution from ZIRC Zebrafish approaches enhance the translational research Feb 01, 2014 · A number of approaches have been developed to overcome the labor associated with identifying specific genetic mutations resulting from random mutagenesis, including large-scale retroviral insertion approaches.110, 115 The result is a large and growing collection of mutant zebrafish lines, many of which are available for distribution from ZIRC

Zebrafish type I collagen mutants faithfully recapitulate

Aug 21, 2018 · Zebrafish with Different Mutations in Type I Collagen Show Variable Skeletal Phenotypes. We systematically analyzed skeletal phenotypes in a large set of zebrafish models carrying different mutations in the zebrafish type I collagen-encoding genes col1a1a, col1a1b, and col1a2 ().As a reference, we also included two knockout mutants, bmp1a / and plod2 /, representing severe zfishbook:Connecting you to a world of zebrafish AB - zfishbook is an internet-based openly accessible database of revertible protein trap gene-breaking transposon (GBT) insertional mutants in the zebrafish, Danio rerio. In these lines, a monomeric red fluorescent protein (mRFP) is encoded by an artificial 3 exon, resulting in a translational fusion to endogenous loci.

zfishbook:connecting you to a world of zebrafish - CORE

zfishbook is an internet-based openly accessible database of revertible protein trap gene-breaking transposon (GBT) insertional mutants in the zebrafish, Danio rerio. In these lines, a monomeric red fluorescent protein (mRFP) is encoded by an artificial 3 exon, resulting in a translational fusion to endogenous loci. zfishbook:connecting you to a world of zebrafish zfishbook is an internet-based openly accessible database of revertible protein trap gene-breaking transposon (GBT) insertional mutants in the zebrafish, Danio rerio. In these lines, a monomeric red fluorescent protein (mRFP) is encoded by an artificial 3 0 exon, resulting in a translational fusion to endogenous loci.

zfishbook:connecting you to a world of zebrafish

zfishbook is an internet-based openly accessible database of revertible protein trap gene-breaking transposon (GBT) insertional mutants in the zebrafish, Danio rerio. In these lines, a monomeric red fluorescent protein (mRFP) is encoded by an artificial 3 0 exon, resulting in a translational fusion to endogenous loci. zfishbook:connecting you to a world of zebrafish zfishbook is an internet-based openly accessible database of revertible protein trap gene-breaking transposon (GBT) insertional mutants in the zebrafish, Danio rerio. In these lines, a monomeric red fluorescent protein (mRFP) is encoded by an artificial 3 exon, resulting in a translational fusion to endogenous loci.

zfishbook:Connecting you to a world of zebrafish revertible mutants Article (PDF Available) in Nucleic Acids Research 40(Database issue):D907-11 · November 2011 with 118

Leave a Comment